PSI-7409

Cat. No.: HY-15745: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (PSI-7409 tetrasodium) that retains the same biological activity.

For research use only. We do not sell to patients.

PSI-7409 Chemical Structure

CAS No. : 1015073-42-3

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of PSI-7409:

PSI-7409 tetrasodium

Other Forms of PSI-7409:

PSI-7409 tetrasodium In-stock

6 Publications Citing Use of MCE PSI-7409

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	> 100 μM Compound: 2'-F,2'-C-Me-UTP	Inhibition of RNA polymerase 2 isolated from human HeLa cell nuclear extract assessed as decrease in incorporation of P32-alpha-CTP after 2 hrs by scintillation counting method Inhibition of RNA polymerase 2 isolated from human HeLa cell nuclear extract assessed as decrease in incorporation of P32-alpha-CTP after 2 hrs by scintillation counting method	[PMID: 28595015]
HeLa	IC₅₀	> 100 μM Compound: 3a-TP	Inhibition of RNA polymerase 2 in human HeLa cell nuclear extract assessed as reduction in RNA transcription by PAGE analysis Inhibition of RNA polymerase 2 in human HeLa cell nuclear extract assessed as reduction in RNA transcription by PAGE analysis	[PMID: 30951311]

In Vitro

PSI-7409 inhibits the enzymatic activities of these NS5BΔ21 polymerases in a dose-dependent manner. The IC₅₀s for PSI-7409 against GT 1b, 2a, 3a, and 4a NS5B polymerases are 1.6 μM, 2.8 μM, 0.7 μM, and 2.6 μM, respectively. PSI-7409 is a weak inhibitor of DNA Pol α (IC₅₀=550 μM). DNA Pol β and γ are not inhibited by 1 mM PSI-7409. A significant amount of RNA product is made in the presence of 500 μM PSI-7409, about 85%^[1]. In clone A cells, the levels of PSI-7409 gradually increases to a maximum concentration of about 25 μm over a period of 48 h. PSI-7409 forms at a much faster rate in primary human hepatocytes, achieving a maximum intracellular concentration of ～100 μM at 4 h and remains at that concentration for 48 h^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

500.16

Formula

C₁₀H₁₆FN₂O₁₄P₃

CAS No.

1015073-42-3

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@@](C)(F)[C@H](N1C(NC(C=C1)=O)=O)O2)[C@H]2COP(O)(OP(OP(O)(O)=O)(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (99.97 mM; Need ultrasonic)

DMF : < 1 mg/mL (insoluble)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9994 mL	9.9968 mL	19.9936 mL
5 mM	0.3999 mL	1.9994 mL	3.9987 mL
10 mM	0.1999 mL	0.9997 mL	1.9994 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.03%

Select Batch:

Data Sheet (274 KB) SDS (392 KB)

COA (249 KB) HNMR (279 KB) LCMS (271 KB)

Handling Instructions (2659 KB)

References

[1]. Lam AM, et al. PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96. [Content Brief]

[2]. Murakami E, et al. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.9994 mL	9.9968 mL	19.9936 mL	49.9840 mL
	5 mM	0.3999 mL	1.9994 mL	3.9987 mL	9.9968 mL
	10 mM	0.1999 mL	0.9997 mL	1.9994 mL	4.9984 mL
	15 mM	0.1333 mL	0.6665 mL	1.3329 mL	3.3323 mL
	20 mM	0.1000 mL	0.4998 mL	0.9997 mL	2.4992 mL
	25 mM	0.0800 mL	0.3999 mL	0.7997 mL	1.9994 mL
	30 mM	0.0666 mL	0.3332 mL	0.6665 mL	1.6661 mL
	40 mM	0.0500 mL	0.2499 mL	0.4998 mL	1.2496 mL
	50 mM	0.0400 mL	0.1999 mL	0.3999 mL	0.9997 mL
	60 mM	0.0333 mL	0.1666 mL	0.3332 mL	0.8331 mL
	80 mM	0.0250 mL	0.1250 mL	0.2499 mL	0.6248 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

PSI-7409 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PSI-74091015073-42-3PSI7409PSI 7409HCVHepatitis C virusInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks