2. Anti-infection
  3. HCV
  4. PSI-7409

PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (PSI-7409 tetrasodium) that retains the same biological activity.

For research use only. We do not sell to patients.

PSI-7409 Chemical Structure

PSI-7409 Chemical Structure

CAS No. : 1015073-42-3

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PSI-7409 Related Antibodies

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Description

PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.

In Vitro

PSI-7409 inhibits the enzymatic activities of these NS5BΔ21 polymerases in a dose-dependent manner. The IC50s for PSI-7409 against GT 1b, 2a, 3a, and 4a NS5B polymerases are 1.6 μM, 2.8 μM, 0.7 μM, and 2.6 μM, respectively. PSI-7409 is a weak inhibitor of DNA Pol α (IC50=550 μM). DNA Pol β and γ are not inhibited by 1 mM PSI-7409. A significant amount of RNA product is made in the presence of 500 μM PSI-7409, about 85%[1]. In clone A cells, the levels of PSI-7409 gradually increases to a maximum concentration of about 25 μm over a period of 48 h. PSI-7409 forms at a much faster rate in primary human hepatocytes, achieving a maximum intracellular concentration of ∼100 μM at 4 h and remains at that concentration for 48 h[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PSI-7409 Related Antibodies
Molecular Weight

500.16

Formula

C10H16FN2O14P3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@@](C)(F)[C@H](N1C(NC(C=C1)=O)=O)O2)[C@H]2COP(O)(OP(OP(O)(O)=O)(O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (99.97 mM; Need ultrasonic)

DMF : < 1 mg/mL (insoluble)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9994 mL 9.9968 mL 19.9936 mL
5 mM 0.3999 mL 1.9994 mL 3.9987 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.9994 mL 9.9968 mL 19.9936 mL 49.9840 mL
5 mM 0.3999 mL 1.9994 mL 3.9987 mL 9.9968 mL
10 mM 0.1999 mL 0.9997 mL 1.9994 mL 4.9984 mL
15 mM 0.1333 mL 0.6665 mL 1.3329 mL 3.3323 mL
20 mM 0.1000 mL 0.4998 mL 0.9997 mL 2.4992 mL
25 mM 0.0800 mL 0.3999 mL 0.7997 mL 1.9994 mL
30 mM 0.0666 mL 0.3332 mL 0.6665 mL 1.6661 mL
40 mM 0.0500 mL 0.2499 mL 0.4998 mL 1.2496 mL
50 mM 0.0400 mL 0.1999 mL 0.3999 mL 0.9997 mL
60 mM 0.0333 mL 0.1666 mL 0.3332 mL 0.8331 mL
80 mM 0.0250 mL 0.1250 mL 0.2499 mL 0.6248 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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PSI-7409 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PSI-74091015073-42-3PSI7409PSI 7409HCVHepatitis C virusInhibitorinhibitorinhibit

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