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  4. RT-AM

RT-AM is a pro-agent real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.

For research use only. We do not sell to patients.

RT-AM Chemical Structure

RT-AM Chemical Structure

CAS No. : 2280796-94-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 2139 In-stock
Solution
10 mM * 1 mL in DMSO USD 2139 In-stock
Solid
1 mg USD 350 In-stock
5 mg   Get quote  
10 mg   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of RT-AM:

RT-AM Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

RT-AM is a pro-agent real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.

In Vitro

To enhance the cell permeability of Real Thiol, the carboxylic acid groups is converted to acetoxymethyl (AM) esters, which are readily hydrolysed by esterases to regenerate Real Thiol inside cells. For cells treated with the RT-AM, real-time ratiometric images of the rapid changes of intracellular GSH concentrations in single cells can be generated by dividing the fluorescence intensity values for the 405 nm channel by the 488 nm channel at each corresponding pixel[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-AM Related Antibodies
Molecular Weight

555.49

Formula

C26H25N3O11
CAS No.
Appearance

Solid

Color

Pink to red

SMILES

O=C1OC2=CC(N3CCC3)=CC=C2C=C1/C=C(C#N)/C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (45.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8002 mL 9.0011 mL 18.0021 mL
5 mM 0.3600 mL 1.8002 mL 3.6004 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.07%

COA (252 KB) HNMR (270 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

The HeLa cell line is grown in DMEM media supplemented with 10% FBS and 1% 1003 Pen Strep. Cells are cultured under a controlled atmosphere (37°C, 5% CO2). Glass bottom dishes are used for cell culture due to confocal scanning requirements. Cells are treated with RT-AM (1 μM with 1% DMSO in DMEM) for 10-15 min before imaging. Confocal images are acquired with 405 nm laser/418–495 nm filter and 488 nm laser/499–615 nm filter. All the microscope settings are kept consistent in each[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8002 mL 9.0011 mL 18.0021 mL 45.0053 mL
5 mM 0.3600 mL 1.8002 mL 3.6004 mL 9.0011 mL
10 mM 0.1800 mL 0.9001 mL 1.8002 mL 4.5005 mL
15 mM 0.1200 mL 0.6001 mL 1.2001 mL 3.0004 mL
20 mM 0.0900 mL 0.4501 mL 0.9001 mL 2.2503 mL
25 mM 0.0720 mL 0.3600 mL 0.7201 mL 1.8002 mL
30 mM 0.0600 mL 0.3000 mL 0.6001 mL 1.5002 mL
40 mM 0.0450 mL 0.2250 mL 0.4501 mL 1.1251 mL
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RT-AM Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RT-AM2280796-94-1Fluorescent DyeInhibitorinhibitorinhibit

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