2. Cell Cycle/DNA Damage Autophagy
  3. Polo-like Kinase (PLK) Autophagy
  4. SBE13

SBE13 is a potent and selective Plk1 inhibitor, with an IC50 of 200 pM; SBE13 poorly inhibits Plk2 (IC50>66 μM) or Plk3 (IC50=875 nM).

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (SBE13 Hydrochloride) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

SBE13 Chemical Structure

SBE13 Chemical Structure

CAS No. : 775294-82-1

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Other In-stock Forms of SBE13:

Other Forms of SBE13:

SBE13 Related Antibodies

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PLK1 PLK2 PLK3 PLK4

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  • Purity & Documentation

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Description

SBE13 is a potent and selective Plk1 inhibitor, with an IC50 of 200 pM; SBE13 poorly inhibits Plk2 (IC50>66 μM) or Plk3 (IC50=875 nM).

IC50 & Target[1]

PLK1

200 pM (IC50)

PLK3

875 nM (IC50)

Cellular Effect
Cell Line Type Value Description References
A-431 EC50
33 μM
Compound: 38; SBE13
Antiproliferative activity against human A-431 cells assessed as reduction in cell proliferation incubated for 72 hrs
Antiproliferative activity against human A-431 cells assessed as reduction in cell proliferation incubated for 72 hrs
[PMID: 35878418]
A549 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
Detroit 562 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human Detroit 562 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human Detroit 562 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
HCT-15 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human HCT-15 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human HCT-15 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
HeLa EC50
18 μM
Compound: 13, SBE13
Antiproliferative activity against human HeLa cells assessed as growth inhibition after 24 to 72 hrs
Antiproliferative activity against human HeLa cells assessed as growth inhibition after 24 to 72 hrs
[PMID: 25304894]
HeLa EC50
18 μM
Compound: 38; SBE13
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
HT-29 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
LN-229 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
MCF7 EC50
12 μM
Compound: 38; SBE13
Antiproliferative activity against human MCF7 cells assessed as reduction in cell proliferation incubated for 72 hrs
Antiproliferative activity against human MCF7 cells assessed as reduction in cell proliferation incubated for 72 hrs
[PMID: 35878418]
PC-3 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
SK-OV-3 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
SKW 6.4 EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human SKW 6.4 cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human SKW 6.4 cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
U2OS EC50
5 μM
Compound: 38; SBE13
Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs
Antiproliferative activity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs
[PMID: 35878418]
In Vitro

SBE13 significantly reduce cell proliferation and induce apoptosis in HeLa cells, with an EC50 of 18 μM[1]. SBE13 (1-100 μM) shows no effect on Caspase 3/7 activity in NIH-3T3 cells. SBE13 (66 and 100 μM) does not change morphology after treatment of primary cells. SBE13 (10 and 100 μM) reduces pRb staining in primary cells, and this indicates a G0/G1 arrest[2]. SBE13 (66 and 100 μM) increases levels of cyclin B1, phospho histone H3, Wee1, Emi1 and securin, and results in cleavage of Cdc27 in HeLa cells. SBE13 (10 and 100 μM) also induces apoptosis of HeLa cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SBE13 Related Antibodies
Molecular Weight

442.94

Formula

C24H27ClN2O4
CAS No.
SMILES

COC1=CC=C(CCNCC2=CC=C(OCC3=CC=C(Cl)N=C3)C(OC)=C2)C=C1OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Kinase Assay
[1]

To assay Plk1 kinase activity, cells are lysed after 13 h release in the presence of SBE13 after double thymidine block and kinase is immunoprecipitated from lysates using antibodies. In brief, for each immunoprecipitation 800 μg of total protein are incubated with Plk1 antibody cocktail (1.5 μg) for 2 h at 4°C on a rotator. Immunoprecipitated protein is collected using Protein A/G Agarose beads. Plk1 immunoprecipitates are incubated with casein (1 μg) and with [γ-32P]ATP (1 μCi) for 30 min at 37°C in kinase buffer. Products from the kinase assays are fractionated on 10 % bis-tris-polyacrylamide gels, and phosphorylated substrate is visualized by autoradiography after an exposure of 12-36 h. Equal amounts of immunoprecipitates are subjected to Western blot analysis to confirm equal loading of Plk1 protein in kinase reactions[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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SBE13 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SBE13775294-82-1SBE 13SBE-13Polo-like Kinase (PLK)AutophagyInhibitorinhibitorinhibit

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