2. Others Apoptosis Metabolic Enzyme/Protease
  3. Fluorescent Dye Apoptosis Mitochondrial Metabolism
  4. Tartrazine

Tartrazine  (Synonyms: Acid Yellow 23; FD&C Yellow No. 5)

Cat. No.: HY-D0257 Purity: 99.78%
SDS
COA
Handling Instructions Technical Support

Tartrazine (Acid Yellow 23; FD&C Yellow No. 5) is an orally active azo acid dye, orange-yellow powder, soluble in water and turns yellow. Tartrazine is mainly used as a synthetic lemon yellow azo dye for food coloring. Tartrazine is the most stable colorant.Tartrazine induces mitochondria-mediated Apoptosis. Tartrazine can cause neurodevelopmental toxicity, cytotoxicity, and genotoxicity.

For research use only. We do not sell to patients.

Tartrazine Chemical Structure

Tartrazine Chemical Structure

CAS No. : 1934-21-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Tartrazine:

Tartrazine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Tartrazine

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tartrazine (Acid Yellow 23; FD&C Yellow No. 5) is an orally active azo acid dye, orange-yellow powder, soluble in water and turns yellow. Tartrazine is mainly used as a synthetic lemon yellow azo dye for food coloring. Tartrazine is the most stable colorant.Tartrazine induces mitochondria-mediated Apoptosis. Tartrazine can cause neurodevelopmental toxicity, cytotoxicity, and genotoxicity[1][2][3][4][5][6][7][8][9][10][11].

In Vitro

Tartrazine (12.5-100 μg/mL, 72 h) shows cytotoxic and mutagenic effects on eukaryotic cells (MN01, FGH)[1].
Tartrazine (5-20 μg/mL) induces chromosomal aberrations in mammalian cells (M. muntjac cells) [3].
Tartrazine (2500  μg/mL, 48 h) showes cytotoxic activity in human lymphocytes due to significant decrease in mitotic index (MI) in the absence of S9 mix[4].
Tartrazine (10-2000 μM, 3-24 h) can be regarded as toxic in CHO cells, with decreasing intracellular GSH, increasing MDA levels)[5].
Tartrazine (0-1000 µg/mL, 6 h) disrupts vascular formation in human primary endothelial cells[8].
Tartrazine (0.25-64.0 mM) has significant genotoxicity, although has no cytotoxic effects[9].
Tartrazine (6.25-800  mg/L, 96 h) induces neurodevelopmental toxicity via mitochondria-mediated apoptosis in zebrafish embryos[10].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tartrazine Related Antibodies

Cell Viability Assay[1]

Cell Line: MN01, FGH
Concentration: 1.56, 3.12, 6.25, 12.5, 25, 50, 100 μg/mL
Incubation Time: 72 h
Result: Showed significant cytotoxic effects on the MN01 from 25 μg/mL to the highest test concentration (100 μg/mL) for MN01 (normal stomach human cell line) and from 12.5 to 100 μg/mL for FGH (normal fibroblast human cell line).
In Vivo

Tartrazine (1-7.5 mg/kg, p.o., 30 days) induces structural and functional aberrations as well as genotoxic effects in rats[2].
Tartrazine (72 mg/kg, p.o., 60 days) is capable of producing free radicals, which in turn cause damage to the cellular compartment system of rat testis[6].
Tartrazine (200 mg/kg, p.o., 60 days) shows diffuse vacuolar degeneration in hepatic parenchyma in rats[7].
Tartrazine (175-700 mg/kg for mice, 125-500 mg/kg for rats, p.o., once a day for 30 d) produces a few adverse effects in learning and memory functions in animals (mice and rats)[11].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

534.36

Formula

C16H9N4Na3O9S2
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=C(C(C1N=NC2=CC=C(S(=O)(O[Na])=O)C=C2)=NN(C3=CC=C(S(=O)(O[Na])=O)C=C3)C1=O)O[Na]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (93.57 mM; Need ultrasonic)

DMSO : 35 mg/mL (65.50 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8714 mL 9.3570 mL 18.7140 mL
5 mM 0.3743 mL 1.8714 mL 3.7428 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.68 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 16.67 mg/mL (31.20 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.85%

SDS (644 KB)

COA (252 KB) HNMR (274 KB) RP-HPLC (222 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.8714 mL 9.3570 mL 18.7140 mL 46.7849 mL
5 mM 0.3743 mL 1.8714 mL 3.7428 mL 9.3570 mL
10 mM 0.1871 mL 0.9357 mL 1.8714 mL 4.6785 mL
15 mM 0.1248 mL 0.6238 mL 1.2476 mL 3.1190 mL
20 mM 0.0936 mL 0.4678 mL 0.9357 mL 2.3392 mL
25 mM 0.0749 mL 0.3743 mL 0.7486 mL 1.8714 mL
30 mM 0.0624 mL 0.3119 mL 0.6238 mL 1.5595 mL
40 mM 0.0468 mL 0.2339 mL 0.4678 mL 1.1696 mL
50 mM 0.0374 mL 0.1871 mL 0.3743 mL 0.9357 mL
60 mM 0.0312 mL 0.1559 mL 0.3119 mL 0.7797 mL
H2O 80 mM 0.0234 mL 0.1170 mL 0.2339 mL 0.5848 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Tartrazine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tartrazine1934-21-0Acid Yellow 23 FD&C Yellow No. 5Acid Yellow23Acid Yellow-23Fluorescent DyeApoptosisMitochondrial Metabolismneurodevelopmental toxicitycytotoxicity genotoxicityCHOMN01FGH.Inhibitorinhibitorinhibit

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