Thioflavine S (Synonyms: Thioflavin S; Direct Yellow 7)

Cat. No.: HY-D0972: FAQs
Data Sheet SDS COA Handling Instructions

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Thioflavine S is a fluorescent histochemical marker of dense core senile plaques. Thioflavine S can be used for Alzheimer's research.

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Thioflavine S Chemical Structure

CAS No. : 1326-12-1

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Based on 17 publication(s) in Google Scholar

17 Publications Citing Use of MCE Thioflavine S

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Description	Thioflavine S is a fluorescent histochemical marker of dense core senile plaques. Thioflavine S can be used for Alzheimer's research^[1].
In Vitro	Thioflavine S staining protocol 1.Deparaffinization and hydration of tissue sections: Place slides in holders and treat with the clearing agent xylene (paraffin solvent) and a series of graded EtOH as follows: 100% xylene - 5 min 100% xylene - 5 min 50%/50% xylene/100% EtOH - 3 min 100% EtOH - 3 min 95% EtOH - 3 min 70% EtOH - 3 min 50% EtOH - 3 min Water - 2 x 3 min Note: Change the solns after about 5 uses or when you see the soln is not cleanly running off the slides, indicating too much paraffin in them. 2.Incubate in filtered 1% aqueous Thioflavine-S for 8 minutes at room temperature (filter Thioflavin-S before each use). Note: protect thioflavin-S from light, and protect the stained slides from light as much as possible. Thioflavin-S stain should be stored at 4℃. 3.Wash 2x 3 min in 80% ethanol 4.Wash 3 min in 95% ethanol 5.Wash with 3 exchanges of distilled water 6.Coverslip in aqueous mounting media and allow slides to dry in the dark overnight. 7.Next day, seal coverslip with clear nail polish. 8.Analyze slides within the next few days-weeks because the staining will fade with time. Store the slides in the dark at 4℃. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Thioflavine S Related Antibodies
CAS No.	1326-12-1
Appearance	Solid
Color	Yellow to brown
SMILES	[Thioflavin S]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility	In Vitro: H₂O : ≥ 50 mg/mL "≥" means soluble, but saturation unknown. Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo: For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution. Protocol 1 Add each solvent one by one: PBS Solubility: 100 mg/mL; Clear solution; Need ultrasonic and warming and heat to 60°C In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: sales@medchemexpress.com Technical Support: tech@medchemexpress.com
Purity & Documentation	Select Batch: Data Sheet (269 KB) SDS (393 KB) COA (241 KB) Handling Instructions (2659 KB)
Dyeing Example	Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to measure the myocardial no-refow phenomenon. Method: For Thioflavine S staining assay. 1. Thioflavine S (1 mL/kg, 6%) is injected into mice via the tail vein and allowed to circulate for 1-2 min. 2. The hearts are quickly harvested, fixed in 4% paraformaldehyde (PFA) overnight, cut into 1 mm sections, and then observed under a stereomicroscope (Leica, Germany). Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to measure the extracellular insoluble Aβ plaque deposits in the whole brain of mice. Method: For brain tissue staining. 1. Wash mouse brain tissues for three times in PBS (5 min). 2. Stain sliced brain tissues with Thioflavine S (500 µM; 8 min; room temperature) solution dissolved in 50% ethanol. 3. Subsequently, after incubating the tissues twice in 50% ethanol for 3 min, the tissues are washed with PBS for 5 min. 4. Brain tissue sections are mounted on glass slides using a TissueFAXS plus (TissueGnostics GmbH, Vienna, Austria) and using a Zeiss Axio Imager Z2 Microscope System (Carl Zeiss, Jena, Germany) for image. Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to evaluate amyloid deposition. Method: For brain tissue staining. 1. Testing sections are immersed in Thioflavine S (1%; 5 min), hydrated in 70% ethanol and washed for 5 min with PBS for twice. 2. The sections are sealed with a fluorescent mounting media. Pannoramic MIDI is used to scan the images and analyze the data. Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker with green fluorescence, it can be used to measure the aggregation of α-syn. Method: For brain sections staining. 1. Stain sections with Thioflavine S solution (10 mg/mL; 5 min). 2. Wash cells for twice. 3. The sections are mounted on slides and images are gathered using a fluorescence microscope (Olympus). Description: Thioflavine S (Thioflavin S) is a fluorescent histochemical marker, it can be used to monitor the accumulation of Aβ. Method: For cell staining. 1. Inoculate cells on a 24-well culture plate and fix cells with 4% paraformaldehyde (15 min; 25℃). 2. Add 80% ethanol to percolate at -20℃ for 6 min. 3. Add Thioflavine S (0.1%; 5 min; 25℃) to percolate for incubate cells. 4. Use the ImageXpress High-content Imaging system for image. Image Pro Plus 6.0 Image analysis software is used to detect the average fluorescence density.
References	[1]. McLellan ME, et al. In vivo imaging of reactive oxygen species specifically associated with thioflavine S-positive amyloid plaques by multiphoton microscopy. J Neurosci. 2003 Mar 15;23(6):2212-7. [Content Brief]

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Thioflavine S1326-12-1Thioflavin S Direct Yellow 7Direct Yellow7Direct Yellow 7Direct Yellow-7Fluorescent DyeFluorescenthistochemicalmarkerAlzheimersenile plaquesInhibitorinhibitorinhibit

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Thioflavine S (Synonyms: Thioflavin S; Direct Yellow 7)

Customer Review

Thioflavine S Related Antibodies

17 Publications Citing Use of MCE Thioflavine S

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