Tomatidine hydrochloride

Name: Tomatidine hydrochloride
Brand: MedChemExpress
SKU: HY-N2149A
Rating: 5.0 (6 reviews)

Cat. No.: HY-N2149A Purity: 99.58%: FAQs
Data Sheet SDS COA Handling Instructions

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Tomatidine hydrochloride acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling. Tomatidine hydrochloride activates autophagy either in mammal cells or C elegans.

For research use only. We do not sell to patients.

Tomatidine hydrochloride Chemical Structure

CAS No. : 6192-62-7

Size	Price	Stock	Quantity
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Tomatidine hydrochloride:

Tomatidine In-stock

5 Publications Citing Use of MCE Tomatidine hydrochloride

: Tomatidine hydrochloride purchased from MedChemExpress. Usage Cited in: FASEB J. 2019 Feb;33(2):2574-2586. [Abstract]; Tomatidine attenuates osteoclastogenesis by inhibiting the MAPK, NF-kB, and PI3K/Akt signaling pathways in vitro.

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All JNK Isoform Specific Products:

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JNK1 JNK2 JNK3 JNK

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Tomatidine hydrochloride acts as an anti-inflammatory agent by blocking NF-κB and JNK signaling^[1]. Tomatidine hydrochloride activates autophagy either in mammal cells or C elegans^[2].

IC₅₀ & Target^[1]

p65

JNK

In Vitro

Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

452.11

Formula

C₂₇H₄₆ClNO₂

CAS No.

6192-62-7

Appearance

Solid

Color

White to yellow

SMILES

[H][C@]1(O[C@]2(NC[C@@H](C)CC2)[C@H]3C)C[C@@]4([H])[C@]5([H])CC[C@@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]13[H].Cl[H]

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

Methanol : 12.5 mg/mL (27.65 mM; Need ultrasonic)

Ethanol : 3.45 mg/mL (7.63 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2119 mL	11.0593 mL	22.1185 mL
5 mM	0.4424 mL	2.2119 mL	4.4237 mL
10 mM	0.2212 mL	1.1059 mL	2.2119 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.58%

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Data Sheet (278 KB) SDS (392 KB)

COA (252 KB) HNMR (287 KB) RP-HPLC (212 KB) MS (80 KB)

Handling Instructions (2659 KB)

References

[1]. Chiu FL, et al. Tomatidine inhibits iNOS and COX-2 through suppression of NF-kappaB and JNK pathways in LPS-stimulated mouse macrophages. FEBS Lett. 2008 Jul 9;582(16):2407-12. [Content Brief]

[2]. Anil Ahsan, et al. Tomatidine Protects Against Ischemic Neuronal Injury by Improving Lysosomal Function. Eur J Pharmacol. 2020 Jun 21;173280. [Content Brief]

Cell Assay
^[1]

RAW 264.7 cells, derived from murine macrophages, are cultured in DMEM supplemented with 10% endotoxin-free, heat-inactivated fetal calf serum, Penicillin (100 units/mL), and Streptomycin (100 μg/mL) in a 5% CO₂ atmosphere at 37 °C in a humidified incubator. For all assay, cell is plated at 2×10⁵ cells/cm² in culture dishes or plates. Treatment with vehicle (0.1% DMSO or 0.1% ethanol), test compounds and/or LPS is carried out under serum-free conditions^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Chiu FL, et al. Tomatidine inhibits iNOS and COX-2 through suppression of NF-kappaB and JNK pathways in LPS-stimulated mouse macrophages. FEBS Lett. 2008 Jul 9;582(16):2407-12. [Content Brief]

[2]. Anil Ahsan, et al. Tomatidine Protects Against Ischemic Neuronal Injury by Improving Lysosomal Function. Eur J Pharmacol. 2020 Jun 21;173280. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / Methanol	1 mM	2.2119 mL	11.0593 mL	22.1185 mL	55.2963 mL
Ethanol / Methanol	5 mM	0.4424 mL	2.2119 mL	4.4237 mL	11.0593 mL
Methanol	10 mM	0.2212 mL	1.1059 mL	2.2119 mL	5.5296 mL
	15 mM	0.1475 mL	0.7373 mL	1.4746 mL	3.6864 mL
	20 mM	0.1106 mL	0.5530 mL	1.1059 mL	2.7648 mL
	25 mM	0.0885 mL	0.4424 mL	0.8847 mL	2.2119 mL