Uridine triphosphate (Synonyms: UTP; Uridine 5'-triphosphate)

Cat. No.: HY-107372: FAQs
Data Sheet SDS Handling Instructions

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Uridine triphosphate (UTP) is a pyrimidine nucleoside triphosphate that is used as a substrate to synthesize RNA or as an energy source in metabolic reactions. Uridine triphosphate activates membrane-bound P2Y2 receptors.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Uridine triphosphate trisodium salt) that retains the same biological activity.

For research use only. We do not sell to patients.

Uridine triphosphate Chemical Structure

CAS No. : 63-39-8

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5 mg	USD 32	Ask For Quote & Lead Time
10 mg	USD 50	Ask For Quote & Lead Time
50 mg	USD 80	Ask For Quote & Lead Time
100 mg	USD 120	Ask For Quote & Lead Time

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Other Forms of Uridine triphosphate:

Uridine triphosphate-¹³C₉,¹⁵N₂ sodium Get quote
Uridine triphosphate-¹³C₉ dilithium Get quote
Uridine triphosphate-d₁₃ dilithium Get quote
Uridine triphosphate-¹⁵N₂ dilithium Get quote
Uridine triphosphate-¹³C₉,¹⁵N₂ dilithium Get quote
Uridine triphosphate trisodium dihydrate Get quote
Uridine 5'-triphosphate tris salt Get quote
Uridine triphosphate trisodium salt Get quote

2 Publications Citing Use of MCE Uridine triphosphate

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	0.45 μM Compound: UTP	Activity against human P2Y2-GFP expressed in A549 cells by intracellular calcium increase Activity against human P2Y2-GFP expressed in A549 cells by intracellular calcium increase	[PMID: 16539385]
HEK293	EC₅₀	0.13 μM Compound: UTP	Activity against rat P2Y2-GFP transfected in HEK293 cells by intracellular calcium increase Activity against rat P2Y2-GFP transfected in HEK293 cells by intracellular calcium increase	[PMID: 16539385]
NG108-15	EC₅₀	1.26 μM Compound: UTP	Agonist activity at mouse P2Y2 receptor expressed in NG108-15 hybrid cells assessed as increase in intracellular calcium release by fluorescence assay Agonist activity at mouse P2Y2 receptor expressed in NG108-15 hybrid cells assessed as increase in intracellular calcium release by fluorescence assay	[PMID: 19523835]
NG108-15	IC₅₀	1.51 μM Compound: UTP	Antagonist activity at mouse P2Y2 receptor expressed in NG108-15 hybrid cells assessed as inhibition of 1 uM UTP-induced intracellular calcium release preincubated 30 mins before UTP challenge by fluorescence assay Antagonist activity at mouse P2Y2 receptor expressed in NG108-15 hybrid cells assessed as inhibition of 1 uM UTP-induced intracellular calcium release preincubated 30 mins before UTP challenge by fluorescence assay	[PMID: 19523835]

In Vitro

Uridine triphosphate (UTP) (0, 10, 100, 250, 500, and 1000 μM; 2, 4, 6, 8, 10, and 12 h) enhances RT4-D6P2T cells migration and wound repair. Uridine triphosphate induces Schwannoma cell migration through activation of P2Y2 receptors and through the increase of extracellular MMP-2 activation and expression^[2].
Uridine triphosphate (UTP) evokes MAPK phosphorylation in a biphasic pattern MAPK phosphorylation, with an early transient phosphorylation 5 min after treatment, and a late and sustained phosphorylation that appeared at 6 h and lasted up to 24 h^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	RT4-D6P2T cells
Concentration:	250 µM
Incubation Time:	5 min, 45 min, 2 h, 6 h, 12 h, 24 h, 48 h
Result:	Induces biphasic MAPK phosphorylation.

In Vivo

Uridine triphosphate (UTP) (0.44-4.4 μg/kg; i.v.; once) reduces infarct size and improves rat heart function after myocardial infarct^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (250-300 g; 2-3 months) with myocardial infarction (MI)^[3]
Dosage:	0.44 µg/kg 30 min before MI, and 4.4 µg/kg i.v. 24 h prior to MI.
Administration:	i.v.; once
Result:	Reduced infarct size and improves rat heart function after myocardial infarct.

Clinical Trial

Molecular Weight

484.14

Formula

C₉H₁₅N₂O₁₅P₃

CAS No.

63-39-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(O)(OP(OP(O)(O)=O)(O)=O)=O)O2)NC(C=C1)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H₂O : ≥ 150 mg/mL (309.83 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0655 mL	10.3276 mL	20.6552 mL
5 mM	0.4131 mL	2.0655 mL	4.1310 mL
10 mM	0.2066 mL	1.0328 mL	2.0655 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (206.55 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Data Sheet (278 KB) SDS (252 KB) Handling Instructions (2659 KB)

References

[1]. Choi JH, et al. Uridine triphosphate increases proliferation of human cancerous pancreatic duct epithelial cells by activating P2Y2 receptor. Pancreas. 2013 May;42(4):680-6. [Content Brief]

[2]. Lamarca A, et al. Uridine 5'-triphosphate promotes in vitro Schwannoma cell migration through matrix metalloproteinase-2 activation. PLoS One. 2014 Jun 6;9(6):e98998. [Content Brief]

[3]. Yitzhaki S, et al. Uridine-5'-triphosphate (UTP) reduces infarct size and improves rat heart function aftermyocardial infarct. Biochem Pharmacol. 2006 Oct 16;72(8):949-55. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.0655 mL	10.3276 mL	20.6552 mL	51.6380 mL
	5 mM	0.4131 mL	2.0655 mL	4.1310 mL	10.3276 mL
	10 mM	0.2066 mL	1.0328 mL	2.0655 mL	5.1638 mL
	15 mM	0.1377 mL	0.6885 mL	1.3770 mL	3.4425 mL
	20 mM	0.1033 mL	0.5164 mL	1.0328 mL	2.5819 mL
	25 mM	0.0826 mL	0.4131 mL	0.8262 mL	2.0655 mL
	30 mM	0.0689 mL	0.3443 mL	0.6885 mL	1.7213 mL
	40 mM	0.0516 mL	0.2582 mL	0.5164 mL	1.2909 mL
	50 mM	0.0413 mL	0.2066 mL	0.4131 mL	1.0328 mL
	60 mM	0.0344 mL	0.1721 mL	0.3443 mL	0.8606 mL
	80 mM	0.0258 mL	0.1291 mL	0.2582 mL	0.6455 mL
	100 mM	0.0207 mL	0.1033 mL	0.2066 mL	0.5164 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Uridine triphosphate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Uridine triphosphate63-39-8UTP Uridine 5'-triphosphateEndogenous MetaboliteDNA/RNA SynthesisP2Y ReceptorInhibitorinhibitorinhibit

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