2. Epigenetics
  3. Protein Arginine Deiminase
  4. AFM-30a

AFM-30a is a potent protein arginine deiminase 2 (PAD2) inhibitor and has excellent PAD2-selectivity. AFM-30a binds to PAD2 with an EC50 value of 9.5 μM. AFM-30a also inhibits H3 citrullination with an EC50 value of 0.4 μM. AFM-30a can be used for the research of certain cancers and a variety of autoimmune diseases including rheumatoid arthritis (RA), multiple sclerosis, lupus, and ulcerative colitis.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (AFM-30a hydrochloride) that retains the same biological activity.

For research use only. We do not sell to patients.

AFM-30a Chemical Structure

AFM-30a Chemical Structure

CAS No. : 2095107-57-4

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AFM-30a Related Antibodies

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Description

AFM-30a is a potent protein arginine deiminase 2 (PAD2) inhibitor and has excellent PAD2-selectivity. AFM-30a binds to PAD2 with an EC50 value of 9.5 μM. AFM-30a also inhibits H3 citrullination with an EC50 value of 0.4 μM. AFM-30a can be used for the research of certain cancers and a variety of autoimmune diseases including rheumatoid arthritis (RA), multiple sclerosis, lupus, and ulcerative colitis[1].

IC50 & Target[1]

PAD2

 

In Vitro

AFM-30a (compound 30a; 25 μM) has good potency to enter HEK293T/PAD2 cells and covalent modify PAD2 with an EC50 of 9.5 μM[1].
AFM-30a shows good ability to inhibit H3 citrullination with an EC50 of 0.4 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AFM-30a Related Antibodies

Cell Viability Assay[1]

Cell Line: HEK293T/PAD2 cells
Concentration: Various concentrations
Incubation Time: 24 h
Result: Exhibited low cytotoxicity for cells.
In Vivo

AFM-30a suppresses NLRP3 signaling and decreases airway remodeling in PAD2-/-transgenic mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

466.51

Formula

C24H27FN6O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C2C(NCC2=CC=C1)=O)N[C@H](C3=NC4=C(N3C)C=CC=C4OC)CCCNC(CF)=N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Purity & Documentation

Purity: ≥96.0%

COA (253 KB)

Handling Instructions (2659 KB)
References
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AFM-30a Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AFM-30a2095107-57-4Protein Arginine DeiminasePeptidylarginine Deiminaseprotein arginine deiminase 2 (PAD2)H3 citrullinationcancerautoimmune diseasesrheumatoid arthritis (RA)multiple sclerosislupusulcerative colitisHEK293T cellsInhibitorinhibitorinhibit

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