2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Alkaline phosphatase, Bovine intestine

Alkaline phosphatase, Bovine intestine  (Synonyms: Apase)

Cat. No.: HY-P2818
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Alkaline phosphatase, Bovine intestine (Apase) is a membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at alkaline pH. Alkaline phosphatase can be used in molecular biology and enzyme-free analysis. Inhibition of tissue nonspecific alkaline phosphatase can block intracellular lipid accumulation.

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Alkaline phosphatase, Bovine intestine Chemical Structure

Alkaline phosphatase, Bovine intestine Chemical Structure

CAS No. : 9001-78-9

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Alkaline phosphatase, Bovine intestine Related Antibodies

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Description

Alkaline phosphatase, Bovine intestine (Apase) is a membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at alkaline pH. Alkaline phosphatase can be used in molecular biology and enzyme-free analysis. Inhibition of tissue nonspecific alkaline phosphatase can block intracellular lipid accumulation[1][2].

In Vitro

Instructions
1. 30 U/μL 10 mM Tris-HCl (pH8.0), 1 mM MgCl2, 50 mM KCl, 0.1 mM ZnCl2 dissolving enzyme, fresh preparation is recommended.
2. Add the following reagents to a 1.5 mL centrifuge tube to dephosphorylate the DNA:
1) 1-20 pmol DNA fragment
2) 1-2 μL Alkaline Phosphatase
3) 5 μL 500 mM Tris-HCl pH 9.0, 10 mM MgCl2
4) Up to 50 μL sterile ddH2O
Note: Single-stranded DNA or DNA with 5' protruding ends is recommended to be incubated at low temperature, and blunt-end DNA or DNA with 3' protruding ends is recommended to be incubated at higher temperatures.
3. Mix well and incubate at 37℃ or 50℃ for 30 min. Or incubate at 37℃ for 15 min, and then incubate at 50℃ for 15 min.
4. Extract twice with phenol/chloroform/isoamyl alcohol (25:24:1).
Note: Before phenol extraction, it is recommended to incubate it at 56℃ for 30 min in a solution containing 5 mM EDTA, 0.5% SDS, and 50 μg/mL Proteinase K to completely inactivate alkaline phosphatase. Incubate at 75℃ for 10 min before phenol extraction for better inactivation effect.
5. Extract with chloroform/isoamyl alcohol (24:1).
6. Add 2.5 μL 3 M NaCl (final concentration 150 mM), 125 μL (2.5 times volume) pre-cooled ethanol, mix well and place at -20℃ for 30-60 min to precipitate DNA.
4 Centrifuge, completely remove ethanol, and dissolve the DNA in an appropriate amount of deionized water (<20 μL).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Alkaline phosphatase, Bovine intestine Related Antibodies
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CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Alkaline phosphatase, Bovine intestine]
Structure Classification
Initial Source

all tissues throughout the human body
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (Need ultrasonic)

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL; Clear solution; Need ultrasonic

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This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

SDS (251 KB)

COA (229 KB)

Handling Instructions (2659 KB)
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Alkaline phosphatase, Bovine intestine Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Alkaline phosphatase, Bovine intestine9001-78-9ApaseEndogenous MetabolitealkalinephosphatasephosphatemonoestershydrolysisenzymeimmunoassayInhibitorinhibitorinhibit

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