alpha-1,3-Galactosyltransferase (a1,3GalT)  (Synonyms: GGTA1)

alpha-1,3-Galactosyltransferase (a1,3GalT) (GGTA1) catalyzes the generation of the α-gal glycan via the transfer of a galactose (Gal) in α1-3 linkage, from a uridyl-diphosphate (UDP) donor onto the N-acetyllactosamine (Galβ1,4GlcNAc-R) of glycoproteins. alpha-1,3-Galactosyltransferase (a1,3GalT) is responsible for the synthesis of the α-galactose (α-Gal) epitope found in most mammalian species^[1].

Product Information
This product is expressed by recombinant E. coli, N-terminal His Tagged, with a molecular weight of 36 kDa. It is used to connect galactose to the non-reducing end of the sugar chain of Galβ1,4GlcOR in an α1,3 connection manner. It is a key enzyme that catalyzes the synthesis of 3-sugar.

Instructions
1. Add substrate according to the reaction system, 10 mM MnCl₂, and buffer salt (such as Tris-HCl) can be appropriately added to adjust the pH to 7.5, and add an appropriate amount of α1,3GalT (according to reaction requirements)
2. After mixing, incubate the sample at 37℃ for 2-4 hours, or overnight.

Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

128449-51-4

Liquid

Colorless to light yellow

[alpha-1,3-Galactosyltransferase(a1,3GalT)]

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sumnima Singh, et al. Loss of α-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis. Cell Host Microbe. 2021 Mar 10;29(3):347-361.e12.  [Content Brief]

  [2]. Anne-Laure Turcot-Dubois, et al. Long-term evolution of the CAZY glycosyltransferase 6 (ABO) gene family from fishes to mammals--a birth-and-death evolution model. Glycobiology. 2007 May;17(5):516-28.  [Content Brief]