Amido Black 10B  (Synonyms: Naphthol Blue Black)

Amido Black 10B (Naphthol Blue Black) is a highly toxic azo dye. Amido Black 10B is genotoxic and mutagenic. Amido Black 10B can cause respiratory problems. Amido Black 10B is used for amino acid dyeing^[1][2][3][4].

Amido Black 10B (0.125-2.0 mg/mL, 3 h) shows genotoxic and mutagenic effect on mammalian cell HepG2^[2].
Amido Black 10B (500 mg/L, 72 h) is decolorized by Lentinus squarrosulus AF5 (decolorization of 70%)^[3].
Amido Black 10B (100-600 mg/L) is decolorized by Bacillus sp. strain AK1^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

616.49

C₂₂H₁₄N₆Na₂O₉S₂

1064-48-8

Solid

Brown to black

O=S(C1=C(/N=N/C2=CC=C([N+]([O-])=O)C=C2)C(N)=C3C(O)=C(/N=N/C4=CC=CC=C4)C(S(=O)(O[Na])=O)=CC3=C1)(O[Na])=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Angelova R, et al. Magnetically modified sheaths of Leptothrix sp. as an adsorbent for Amido black 10B removal. Journal of Magnetism and Magnetic Materials, 2017, 427: 314-319.

  [2]. Carvalho da Cruz Brambilla CM, et al. Amido Black 10B a widely used azo dye causes DNA damage in pro- and eukaryotic indicator cells. Chemosphere. 2019 Feb;217:430-436.

  [3]. Mathur A, et al. Bioremediation of Textile Azo Dyes Amido Black 10B, Reactive Black 5, Reactive Blue 160 by Lentinus squarrosulus AF5 and Assessment of Toxicity of the Degraded Metabolites. Advances in Microbiology, 2024, 14(2): 137-161.

  [4]. Masarbo R S, et al. Eco-friendly degradative decolourisation and detoxification of azo dye Amido Black 10B by Bacillus sp. strain AK1. Chemistry and Ecology, 2021, 37(7): 668-682.