Amorfrutin B

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Amorfrutin B is a highly potent natural peroxisome proliferation-activated receptor γ (PPARγ) agonist with oral activity with K_i values of 19 nM and EC₅₀ values of 73 nM, respectively. Amorfrutin B has hypoglycemic and neuroprotective activities.

For research use only. We do not sell to patients.

Amorfrutin B Chemical Structure

CAS No. : 78916-42-4

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	0.05 μM Compound: amorfrutin B	Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay	[PMID: 23286787]
HEK293	EC₅₀	73 nM Compound: 2	Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay	[PMID: 25938459]
HT-29	IC₅₀	20 μM Compound: 1	Antiproliferative activity against human HT-29 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay Antiproliferative activity against human HT-29 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay	[PMID: 26731300]
HT-29	IC₅₀	30.5 μM Compound: 2	Reduction in TNFalpha-induced PPARgamma expression in human HT-29 cells after 48 hrs by Western blotting analysis Reduction in TNFalpha-induced PPARgamma expression in human HT-29 cells after 48 hrs by Western blotting analysis	[PMID: 25938459]
MCF7	IC₅₀	33 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay Antiproliferative activity against human MCF7 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay	[PMID: 26731300]
PC-3	IC₅₀	32 μM Compound: 1	Antiproliferative activity against human PC3 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay Antiproliferative activity against human PC3 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay	[PMID: 26731300]
T84	IC₅₀	13 μM Compound: 1	Antiproliferative activity against human T84 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay Antiproliferative activity against human T84 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay	[PMID: 26731300]

In Vitro

Amorfrutin B (1,5 μM, 6 h) inhibits apoptosis and autophagy by gene methylation and miRNA-dependent regulation, and protects mouse brain neurons from hypoxia/ischemia^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Amorfrutin B (100 mg/kg, added to food for 2 weeks) improves insulin sensitivity, glucose tolerance and lipid concentration in insulin-resistant mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	High-fat diet mice model^[2].
Dosage:	100 mg/kg
Administration:	Add to food，2 weeks
Result:	Reduced fasting plasma levels of triacylglycerols by 25% and the concentrations of deleterious plasma NEFA by 29%.

Molecular Weight

408.53

Formula

C₂₆H₃₂O₄

CAS No.

78916-42-4

SMILES

O=C(O)C1=C(CCC2=CC=CC=C2)C=C(OC)C(C/C=C(C)/CC/C=C(C)\C)=C1O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Przepiórska K, et al. Amorfrutin B Protects Mouse Brain Neurons from Hypoxia/Ischemia by Inhibiting Apoptosis and Autophagy Processes Through Gene Methylation- and miRNA-Dependent Regulation. Mol Neurobiol. 2023 Feb;60(2):576-595. [Content Brief]

[2]. Weidner C, et al. Amorfrutin B is an efficient natural peroxisome proliferator-activated receptor gamma (PPARγ) agonist with potent glucose-lowering properties. Diabetologia. 2013 Aug;56(8):1802-12. [Content Brief]

Amorfrutin B Related Classifications

Neurological Disease Metabolic Disease

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amorfrutin B78916-42-4PPARPeroxisome proliferator-activated receptorsPPARγHFDHypoglycemicNeuroprotectiveInhibitorinhibitorinhibit

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