AMP-Deoxynojirimycin (Synonyms: AMP-DNM)

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AMP-Deoxynojirimycin (AMP-DNM) is a potent ceramide glucosyltransferase and GCase 2 inhibitor. AMP-Deoxynojirimycin also is a GlcCer biosynthesis inhibitor.

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AMP-Deoxynojirimycin Chemical Structure

CAS No. : 216758-20-2

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Description

AMP-Deoxynojirimycin (AMP-DNM) is a potent ceramide glucosyltransferase and GCase 2 inhibitor. AMP-Deoxynojirimycin also is a GlcCer biosynthesis inhibitor^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	16500 nM Compound: 6, AMP-DNM	Inhibition of GCS in human A549 cells assessed as decrease in GM1 synthesis after 72 hrs by Fluorescence assay Inhibition of GCS in human A549 cells assessed as decrease in GM1 synthesis after 72 hrs by Fluorescence assay	[PMID: 22497444]
A549	IC₅₀	16500 nM Compound: 5, AMP-DNM	Inhibition of GCS activity in human A549 cells assessed as amount of GM1 on the cell membrane after 72 hrs by FL-CTB-based fluorescent microscopy Inhibition of GCS activity in human A549 cells assessed as amount of GM1 on the cell membrane after 72 hrs by FL-CTB-based fluorescent microscopy	[PMID: 21974949]
CuFi-1	IC₅₀	1.84 nM Compound: 3; D-AMP-DNM	Anti-inflammatory activity in human CuFi1 cells assessed as reduction in Pseudomonas aeruginosa PAO1-induced IL-8 mRNA expression level pretreated for 1 hr followed by Pseudomonas aeruginosa PAO1 challenge and measured after 4 hrs by RT-PCR analysis Anti-inflammatory activity in human CuFi1 cells assessed as reduction in Pseudomonas aeruginosa PAO1-induced IL-8 mRNA expression level pretreated for 1 hr followed by Pseudomonas aeruginosa PAO1 challenge and measured after 4 hrs by RT-PCR analysis	[PMID: 31075609]
RAW	IC₅₀	0.2 μM Compound: 3, AMP-DNM	Inhibition of glucosylceramide synthase in mouse RAW cells preincubated with compound for 15 mins by in-situ enzyme inhibition assay Inhibition of glucosylceramide synthase in mouse RAW cells preincubated with compound for 15 mins by in-situ enzyme inhibition assay	[PMID: 24900289]
RAW	IC₅₀	0.2 μM Compound: 2, AMP-DNM	Inhibition of glucosylceramide synthase in mouse RAW cells preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence scanner Inhibition of glucosylceramide synthase in mouse RAW cells preincubated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence scanner	[PMID: 24900342]
SH-SY5Y	IC₅₀	0.003 μM Compound: 3; D-AMP-DNM	Inhibition of NLGase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of AMP-DNM by fluorescence based assay Inhibition of NLGase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of AMP-DNM by fluorescence based assay	[PMID: 31075609]

In Vitro

AMP-Deoxynojirimycin (10 μM) significantly blocks the leucine and C17ISO-induced P70S6K phosphorylation in 3T3-L1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

397.55

Formula

C₂₂H₃₉NO₅

CAS No.

216758-20-2

SMILES

OC[C@H]([C@H]([C@@H]1O)O)N(C[C@@H]1O)CCCCCOCC2(C[C@H](C3)C4)C[C@H]4C[C@H]3C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhu M, et al. Monomethyl branched-chain fatty acid mediates amino acid sensing upstream of mTORC1. Dev Cell. 2021 Oct 11;56(19):2692-2702.e5. [Content Brief]

[2]. Bae EJ, et al. Loss of glucocerebrosidase 1 activity causes lysosomal dysfunction and α-synuclein aggregation. Exp Mol Med. 2015 Mar 27;47(3):e153. [Content Brief]

AMP-Deoxynojirimycin Related Classifications

Neurological Disease

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMP-Deoxynojirimycin216758-20-2AMP-DNMGlucosidaseGlucosylceramide Synthase (GCS)ceramide glucosyltransferaseGCase 2GlcCer biosynthesis3T3-L1 cellsP70S6KC17ISOInhibitorinhibitorinhibit

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