Antibacterial agent 271 

Antibacterial agent 271 is an antibacterial agent with significant inhibition against Escherichia coli (MIC: 2.2 μM). Antibacterial agent 271 reduces metabolic activity by disrupting the integrity of bacterial membranes. Antibacterial agent 271 binds to DNA grooves to inhibit replication and induces accumulation of reactive oxygen species (ROS) , ultimately leading to bacterial death. Nrf2 activator 19 shows significant potential in combating bacterial infections^[1].

Antibacterial agent 271 (Compound 11c) has an outstanding inhibitory effect on Escherichia coli (MIC: 2.2 μM), and exhibits strong inhibitory effects on other Gram-negative bacteria (Pseudomonas aeruginosa MIC: 17.6 μM) and some Gram-positive bacteria (Staphylococcus aureus MIC: 8.8 μM and Enterococcus faecalis MIC: 4.4 μM)^[1].
Antibacterial agent 271 (2.2 μM-8.8 μM, 4 h) can completely inhibit bacterial growth at 2.2 μM, indicating that it has a good ability to quickly inhibit the growth of Escherichia coli^[1].
Antibacterial agent 271 demonstrates stable MIC values against Escherichia coli after 16 generations of passage experiments, indicating a low risk of resistance development^[1].
Antibacterial agent 271 (0.5632 mM, 24 h) exhibits low hemolytic activity in human red blood cells (RBC)^[1].
Antibacterial agent 271 (40 μM) shows very low cytotoxicity to normal human liver cells (LO2) and breast cancer cells (MCF-7) (cell survival rate>85%)^[1].
Antibacterial agent 271 (2.2 μM -35.2 μM) causes membrane potential imbalance, outer membrane destruction, inner membrane damage and protein leakage in Escherichia coli^[1].
Antibacterial agent 271 (2.2 μM -35.2 μM) inhibits the metabolic activity of Escherichia coli, resulting in loss of cell viability, and induces ROS production in Escherichia coli, thereby inhibiting bacterial growth^[1].
Antibacterial agent 271 (2.2 μM -35.2 μM) interacts with DNA through groove binding, inhibits replication but does not cut DNA, and ultimately destroys the normal biological functions of bacteria^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

455.44

C₂₀H₁₇N₅O₆S

NC1=CC=C(S(=O)(NC2=CC=C(C(CN3C(C)=NC=C3[N+]([O-])=O)=CC(O4)=O)C4=C2)=O)C=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wang YX, et al. Benzo-α-pyrone-derived multitargeting actions to enhance the antibacterial performance of sulfanilamides against Escherichia coli. Bioorg Chem. 2025 May;158:108339.  [Content Brief]