AFP Antibody (YA914)

AFP Antibody (YA914) is an unconjugated, approximately 65 kDa, mouse-derived, anti-AFP (YA914) monoclonal antibody. AFP Antibody (YA914) can be used for: WB, ELISA, IHC-P, IHC-F, IF expriments in human, rat, background without labeling.

174

Predicted band size: 65kDa

affinity purified

Secreted.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Rat,

P02771

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500

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  Western blot analysis of extracts from HepG2 (lane 1) using AFP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-mouse IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, ICC/IF

Liquid

Supplied in 0.01M PBS(pH7.4) with 0.03% Proclin300.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

AFP protein: full length