Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L

Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&Lis an -conjugated, goat-derived anti-mouse IgG antibody. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L conjugates the light and heavy chains of mouse IgG antibodies for use in IF-Cell, IF-Tissue experiments in the mouse context.

Alexa Fluor® 647

Unmodified

Secondary Antibody; Goat Polyclonal Antibody

Polyclonal

Goat

Mouse

IF-Cell: 1:500-1:1,000 ;IF-Tissue: 1:500-1:1,000

• 
  Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
• 
  Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81250) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81250) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P81013) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

ICC/IF

Liquid

Stored at -20°C for 1 year.Protect from light. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous