Annexin A1 Antibody (YA832)

Annexin A1 Antibody (YA832) is a non-conjugated and Mouse origined monoclonal antibody about 39 kDa, targeting to Annexin A1 (5E4D8). It can be used for WB assays with tag free, in the background of Human, Mouse, Rat, Monkey.

Annexin A1 (5E4D8): This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

Free

301

Predicted band size: 39 kDa

affinity purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Monkey

P04083

WB: 1:500-1:1,000

• 
  Western blot analysis of extracts from A549(lane 2(20μg) 、Hela (lane 3（20μg) and C6(lane 4(20μg)), using Annexin A1 (HY-P80544) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80544, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human Annexin A1 beta.The exact sequence is proprietary to MCE.