Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L

Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L is a red Alexa Fluor® 647-conjugated and Goat origined monoclonal antibody, targeting to Rabbit IgG antibody. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L can binds to the light and heavy chains of Rabbit IgG antibodies, thus can be used for ICC/IF, IHC-F, FC, ELISA assays in the background of Rabbit.

Affinity Purified

Alexa Fluor® 647

Unmodified

Secondary Antibody

Polyclonal

Goat

Rabbit

IF: 1:100-1:1,000 ;FC: 1:500-1:1,000 ;ELISA: 1:5000-1:20000

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  Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
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  Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

ICC/IF, IHC-F, FC, ELISA

Liquid

Supplied in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

Stored at -20°C for 1 year.Protect from light. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Endogenous

Synthetic peptide corresponding to Rabbit IgG.