APG5L Antibody

APG5L Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 32 kDa, targeting to APG5L. It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

APG5L: The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Free

9474

Predicted band size: 32 kDa；Observed band size: 55 kDa

affinity purified

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

Q9H1Y0

WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20

• 
  Western blot analysis of extracts from Raji(lane 2(20μg) ), Hela(lane 3(20μg) ) ,RAW264.7(lane 4(20μg) ) and NIH3T3(lane 5(20μg)) using APG5L(HY-P80545) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80545, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human APG5L/ATG5.The exact sequence is proprietary to MCE.