ATP Citrate Lyase Antibody (YA829)

ATP Citrate Lyase Antibody (YA829) is a non-conjugated and Mouse origined monoclonal antibody about 121 kDa, targeting to ATP Citrate Lyase (3D9). It can be used for WB,ICC/IF,FC assays with tag free, in the background of Human, Mouse, Monkey.

ATP Citrate Lyase (3D9): ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Free

47

Predicted band size: 121 kDa;Observed band size: 121 kDa

affinity purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Monkey

P53396

WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;FC: 1:50-1:100

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  Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using ATP Citrate Lyase(HY-P80560) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80560, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, FC

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Synthetic peptide corresponding to Human ATP-Citrate Lyase.The exact sequence is proprietary to MCE.