Beta Actin Antibody

Beta Actin Antibody is a non-conjugated and Mouse origined monoclonal antibody about 42 kDa, targeting to Beta Actin. It can be used for WB,ICC,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

Free

Predicted band size: 42 kDa

Protein A affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

Isotype/Loading Controls

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

SwissProt: P60709 Human;SwissProt: P60710 Mouse;SwissProt: P60711 Rat

WB: 1:10,000; ICC: 1:100-1:200 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg) using Beta Tubulin (HY-P80487) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80487, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human Beta-actin.The exact sequence is proprietary to MCE.