c-Myc Antibody (YA3476)

c-Myc Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 49 kDa, targeting to c-Myc. It can be used for WB, IHC-P, IP, ChIP assays with tag free, in the background of Human, Mouse, Rat.

c-Myc: This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

4609

Predicted band size: 49 kDa;Observed band size: 55 kDa

affinity purified

Nucleoplasm, nucleolus

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human, Mouse, Rat

SwissProt: P01106 Human;SwissProt: P01108 Mouse;SwissProt: P09416 Rat

WB: 1:1,000;IHC-P: 1:200;IP: 1-2μg/sample;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

WB, IHC-P, IP, ChIP

HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Jurkat cell lysate, Daudi cell lysate, K562 cell lysate, HL-60 cell lysate, human B lymphocytoma tissue, human colon tissue

Liquid

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide.