CaMK1 Antibody

CaMK1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 41 kDa, targeting to CaMK1. It can be used for WB assays with tag free, in the background of Mouse, Rat.

CaMK1: Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]

Free

8536

Predicted band size: 41 kDa;Observed band size: 41 kDa

affinity purified

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Mouse, Rat

Q14012

WB: 1:500-1:1,000

• 
  Western blot analysis of extracts from HEK-293(lane 2(20μg) or lane 3(40μg) ) and NIH3T3(lane 4(20μg) or lane 5(40μg) ) using CaMK1(HY-P80580) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80580, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human CaMKI.The exact sequence is proprietary to MCE.