CB1 Antibody

CB1 Antibody is a rabbit-derived non-conjugated IgG antibody, targeting CB1, with a predicted molecular weight of 52 kDa (observed band size: 52 kDa). CB1 Antibody can be used for WB, IHC-P, IHC-F, ICC/IF, ELISA experiments in human, rat backgrounds.

1268

Predicted band size: 52 kDa;Observed band size: 52 kDa

Affinity Chromatography

Cell membrane.

Non-conjugated

Unmodified

Neuroscience

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Rat

P21554

WB: 1:500-2000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500;ELISA: 1:5000-10000;

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  Western blot analysis of extracts from Hela (lane 1) and M-liver (lane 2) using CB1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, IHC-F, ICC/IF, ELISA

Liquid

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human CNR-1: 401-472/472