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CD163 Antibody

Cat. No.: HY-P81177
COA User Guide for Antibodies

CD163 Antibody is an unconjugated, approximately 130 kDa, rabbit-derived, anti-CD163 polyclonal antibody. CD163 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, mouse, and predicted: rat, dog, pig, horse background without labeling.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CD163 Antibody is an unconjugated, approximately 130 kDa, rabbit-derived, anti-CD163 polyclonal antibody. CD163 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, mouse, and predicted: rat, dog, pig, horse background without labeling.

Gene ID

9332

Synonyms
Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN.
Molecular Weight

Predicted band size: 130kDa

Purity

affinity purified

Subcellular Location

Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.

Conjugation

Non-conjugated

Modification

Unmodified

Product Categories

Primary Antibody; Rabbit Polyclonal Antibody

Clonality

Polyclonal

Host

Rabbit

Reactivity

Human, Mouse

Predicted Reactivity

Rat, Dog, Pig, Horse

Note: The predicted reactivity is for reference only, not as a product performance guarantee.
SwissProt ID

Q86VB7

Dilution Ratio

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 1ug/Test;ICC: 1:100-500;IF: 1:100-500

  • Western blot analysis of extracts from RAW264.7 using CD163 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

KLH conjugated synthetic peptide derived from human CD163: 1001-1121/1156

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD163 Antibody
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HY-P81177
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