CD8 Antibody

Cat. No.: HY-P81148: FAQs
Data Sheet SDS COA User Guide for Antibodies

CD8 Antibody is an unconjugated, approximately 27 kDa, rabbit-derived, anti-CD8 polyclonal antibody. CD8 Antibody can be used for: WB, IHC-P, IHC-F, IF expriments in human, mouse, background without labeling.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$75	In-stock	Estimated Time of Arrival: December 31
50 μL	$190	In-stock	Estimated Time of Arrival: December 31
100 μL	$310	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	CD8 Antibody is an unconjugated, approximately 27 kDa, rabbit-derived, anti-CD8 polyclonal antibody. CD8 Antibody can be used for: WB, IHC-P, IHC-F, IF expriments in human, mouse, background without labeling.
Gene ID	12525
Synonyms	CD8 antigen, alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen.
Molecular Weight	Predicted band size: 27kDa
Purity	affinity purified
Subcellular Location	Membrane; Single-pass type I membrane protein.
Conjugation	Non-conjugated
Modification	Unmodified
Product Categories	Primary Antibody; Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Mouse
Predicted Reactivity	Human Note: The predicted reactivity is for reference only, not as a product performance guarantee.
SwissProt ID	P01731
Dilution Ratio	WB: 1:500-2000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500 Western blot analysis of extracts from M-Spleen (lane 1) using CD8 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application	WB, IHC-P, ICC/IF
Appearance	Liquid
Formulation	Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	KLH conjugated synthetic peptide derived from mouse CD8: 51-150/247
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (190 KB) User Guide for Antibodies (1077 KB)

CD8 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD8 Antibody

Biological Activity

Technical Parameters

Properties

Documentation

CD8 Antibody Related Classifications

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