Cyclin B1 Antibody

Cat. No.: HY-P80632: FAQs
Data Sheet SDS COA User Guide for Antibodies

Cyclin B1 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 48 kDa, targeting to Cyclin B1. It can be used for WB,IHC-F,IHC-P,ICC/IF,ELISA assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	Cyclin B1 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 48 kDa, targeting to Cyclin B1. It can be used for WB,IHC-F,IHC-P,ICC/IF,ELISA assays with tag free, in the background of Human, Mouse, Rat.
Background	Cyclin B1: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
Tag	Free
Gene ID	891
Synonyms	CCNB1; CCNB; G2/mitotic-specific cyclin-B1
Molecular Weight	Predicted band size: 48 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Modification	Unmodified
Research Field	Cell Biology
Product Categories	Primary Antibody; Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
SwissProt ID	P14635
Dilution Ratio	WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;ELISA: 1:10,000 Western blot analysis of extracts from Ramos (lane 1) and Hela (lane 2) and Raji (lane 3) and Jurkat (lane 4) using Cyclin B1antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application	WB, IHC-F, IHC-P, ICC/IF, ELISA
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Human Cyclin B1.AA range:91-140
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Cyclin B1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cyclin B1 Antibody

Biological Activity

Technical Parameters

Properties

Documentation

Cyclin B1 Antibody Related Classifications

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