Cytokeratin 19 Antibody

Cat. No.: HY-P81183: FAQs
Data Sheet SDS COA User Guide for Antibodies

Cytokeratin 19 Antibody is an unconjugated, approximately 44 kDa, rabbit-derived, anti-Cytokeratin 19 polyclonal antibody. Cytokeratin 19 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, IF expriments in human and predicted: mouse, rat, chicken, dog, pig, cow, horse, rabbit background without labeling.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Description	Cytokeratin 19 Antibody is an unconjugated, approximately 44 kDa, rabbit-derived, anti-Cytokeratin 19 polyclonal antibody. Cytokeratin 19 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, IF expriments in human and predicted: mouse, rat, chicken, dog, pig, cow, horse, rabbit background without labeling.
Gene ID	3880
Synonyms	40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd.
Molecular Weight	Predicted band size: 44kDa
Purity	affinity purified
Conjugation	Non-conjugated
Modification	Unmodified
Product Categories	Primary Antibody; Rabbit Polyclonal Antibody
Clonality	Polyclonal
Host	Rabbit
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Chicken, Pig, Cow, Dog, Horse Note: The predicted reactivity is for reference only, not as a product performance guarantee.
SwissProt ID	P08727
Dilution Ratio	WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 1μg /Test;IF: 1:100-500 Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Application	WB, ELISA, IHC-P, IHC-F, FC, ICC/IF
Appearance	Liquid
Formulation	Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	KLH conjugated synthetic peptide derived from human Cytokeratin 19.: 101-200/403
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

Cytokeratin 19 Antibody Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:: Cytokeratin 19 Antibody
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Immunofluorescence analysis of MCF7 cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Immunofluorescence analysis of HEC1A cells labeling Cytokeratin 19 with Cytokeratin 19 (HY-P81183) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA/10% normal goat serum for 1 hour at room temperature. Cells were then incubated with Cytokeratin 19 (HY-P81183) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Alexa Fluor® 647-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P80952) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

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Cytokeratin 19 Antibody

Biological Activity

Technical Parameters

Properties

Documentation

Cytokeratin 19 Antibody Related Classifications

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