1. Antibodies
  2. Primary Antibodies
  3. Monoclonal Antibodies Flow Cytometry Antibodies
  4. DNMT1 Antibody (YA781)

DNMT1 Antibody (YA781)

Cat. No.: HY-P80105
COA User Guide for Antibodies

DNMT1 Antibody (YA781) is a non-conjugated and Mouse origined monoclonal antibody about 183 kDa, targeting to DNMT1. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

DNMT1 Antibody (YA781) is a non-conjugated and Mouse origined monoclonal antibody about 183 kDa, targeting to DNMT1. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human.

Tag

Free

Molecular Weight

Predicted band size: 183 kDa

Purity

Protein G affinity purified.

Subcellular Location

Nucleus.

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Epigenetics and Nuclear Signaling

Product Categories

Primary Antibody; Mouse Monoclonal Antibody

Clonality

Monoclonal

Host

Mouse

Reactivity

Human

SwissProt ID

SwissProt: P26358 Human

Dilution Ratio

WB: 1:500-1:2,000;IHC-P: 1:50-1:200;FC: 1:50-1:100

  • Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using DNMT1 (HY-P80105) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80105, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application

WB, IHC-P, FC

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG1

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human Dnmt1.AA range:500-600.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
DNMT1 Antibody (YA781)
Cat. No.:
HY-P80105
Quantity:
MCE Japan Authorized Agent: