eIF4A1 Antibody (YA774)

eIF4A1 Antibody (YA774) is a non-conjugated and Mouse origined monoclonal antibody about 46 kDa, targeting to eIF4A1 (6D7). It can be used for WB,IHC-F,IHC-P,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

eIF4A1 (6D7): Enables double-stranded RNA binding activity. Predicted to be involved in cytoplasmic translational initiation. Located in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]

Free

1973

Predicted band size: 46 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

P60842

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using eIF4A1 Antibody (YA774) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-F, IHC-P, ICC/IF

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to eIF4A1.The exact sequence is proprietary to MCE.