eIF4B Antibody

eIF4B Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 69 kDa, targeting to eIF4B. It can be used for WB,IHC-P assays with tag free, in the background of Human.

eIF4B: Enables RNA binding activity. Predicted to be involved in eukaryotic translation initiation factor 4F complex assembly and formation of translation preinitiation complex. Located in cytosol. Biomarker of autism spectrum disorder and major depressive disorder. [provided by Alliance of Genome Resources, Apr 2022]

Free

1975

Predicted band size: 69 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P23588

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Western blot analysis of extracts from A549(lane 2(20μg) or lane 3(40μg)) and Hela(lane 4(20μg) or lane 5(40μg)), using eIF4B (HY-P80651) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80651, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human eIF4B.The exact sequence is proprietary to MCE.