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  4. eIF4EBP1 Antibody

eIF4EBP1 Antibody

Cat. No.: HY-P80119
COA User Guide for Antibodies

eIF4EBP1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 13 kDa, targeting to eIF4EBP1. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

eIF4EBP1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 13 kDa, targeting to eIF4EBP1. It can be used for WB,ICC/IF,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse, Rat.

Tag

Free

Molecular Weight

Predicted band size: 13 kDa

Purity

Protein A affinity purified.

Subcellular Location

Cytoplasm, cytosol.

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Epigenetics and Nuclear Signaling

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

SwissProt ID

SwissProt: Q13541 Human;SwissProt: Q60876 Mouse;SwissProt: Q62622 Rat

Dilution Ratio

WB: 1:1,000-1:2,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

  • Western blot analysis of extracts from Hela (lane 1) and Raji (lane 2) and K562 (lane 3) using eIF4EBP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, ICC/IF, IHC-P, IP, FC

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human eIF4EBP1.AA range:1-49.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
eIF4EBP1 Antibody
Cat. No.:
HY-P80119
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