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GPR30 Antibody

Cat. No.: HY-P81150
COA User Guide for Antibodies

GPR30 Antibody is an unconjugated, approximately 42 kDa, rabbit-derived, anti-GPR30 polyclonal antibody. GPR30 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, mouse, rat, and predicted: chicken, cow, horse, rabbit background without labeling.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $85 In-stock
50 μL $220 In-stock
100 μL $350 In-stock
250 μL   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

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Description

GPR30 Antibody is an unconjugated, approximately 42 kDa, rabbit-derived, anti-GPR30 polyclonal antibody. GPR30 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, mouse, rat, and predicted: chicken, cow, horse, rabbit background without labeling.

Background

The related gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. The receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. The protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. The receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function.

Gene ID

2852

Synonyms
G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER.
Molecular Weight

Predicted band size: 42kDa

Purity

affinity purified

Subcellular Location

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Golgi apparatus membrane; Multi-pass membrane protein (By similarity). Note=Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.

Conjugation

Non-conjugated

Modification

Unmodified

Product Categories

Primary Antibody; Rabbit Polyclonal Antibody

Clonality

Polyclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

Predicted Reactivity

Chicken, Cow, Horse, Rabbit

Note: The predicted reactivity is for reference only, not as a product performance guarantee.
SwissProt ID

Q99527

Dilution Ratio

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 1µg/Test;ICC: 1:100;IF: 1:100-500

Application

WB, IHC-P, IHC-F, ICC/IF, FC, ELISA

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

KLH conjugated synthetic peptide derived from human GPR30: 251-375/375

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GPR30 Antibody
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HY-P81150
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