HDAC2 Antibody (YA742)

HDAC2 Antibody (YA742) is a non-conjugated and Mouse origined monoclonal antibody about 55 kDa, targeting to HDAC2 (2D9). It can be used for WB,ICC/IF assays with tag free, in the background of Human, Mouse, Rat, Monkey.

HDAC2 (2D9): This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Free

3066

Predicted band size: 55 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Monkey

Q92769

WB: 1:500-1:1,000 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from THP-1(lane 2(20μg) and THP-1(lane 3(40μg), SH-SY5Y(lane 4(20μg) ) and SH-SY5Y(lane 5(40μg) ) using HDAC2 (HY-P80699) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80699, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF

Liquid

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Endogenous

Synthetic peptide corresponding to Human HDAC2.The exact sequence is proprietary to MCE.