Histone H3 (acetyl K27) Antibody

Cat. No.: HY-P80169: FAQs
Data Sheet SDS COA User Guide for Antibodies

Histone H3 (acetyl K27) Antibody is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to Histone H3 (acetyl K27). It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	Histone H3 (acetyl K27) Antibody is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to Histone H3 (acetyl K27). It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.
Tag	Free
Molecular Weight	Predicted band size: 15 kDa
Purity	Protein G affinity purified.
Subcellular Location	Nucleus, Chromosome.
Conjugation	Non-conjugated
Modification	Acetylated
Research Field	Epigenetics and Nuclear Signaling
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Clonality	Monoclonal
Host	Mouse
Reactivity	Human, Mouse, Rat
SwissProt ID	SwissProt: P68431 Human;SwissProt: P84243 Human;SwissProt: Q16695 Human;SwissProt: Q6NXT2 Human;SwissProt: Q71DI3 Human;SwissProt: P68433 Mouse;SwissProt: Q6LED0 Rat
Dilution Ratio	WB: 1:500-1:2,000 ;ICC: 1:100 ;IHC-P: 1:600 ;FC: 1:500 Western blot analysis of extracts from Hela (lane 1) and Jurkat (lane 2) using Histone H3 (acetyl K27) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application	WB, ICC/IF, IHC-P, FC
Appearance	Liquid
Formulation	Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG1
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Human Histone H3.AA range:20-40.
Documentation	Select Batch: Data Sheet (233 KB) SDS (251 KB) COA (191 KB) User Guide for Antibodies (1077 KB)

Histone H3 (acetyl K27) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Histone H3 (acetyl K27) Antibody

Biological Activity

Technical Parameters

Properties

Documentation

Histone H3 (acetyl K27) Antibody Related Classifications

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