1. Antibodies
  2. Primary Antibodies
  3. Monoclonal Antibodies
  4. HSP60 Antibody

HSP60 Antibody

Cat. No.: HY-P80184
COA User Guide for Antibodies

HSP60 Antibody is a non-conjugated and Mouse origined monoclonal antibody about 61 kDa, targeting to HSP60. It can be used for WB,ICC,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

HSP60 Antibody is a non-conjugated and Mouse origined monoclonal antibody about 61 kDa, targeting to HSP60. It can be used for WB,ICC,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

Tag

Free

Molecular Weight

Predicted band size: 61 kDa

Purity

Protein A affinity purified.

Subcellular Location

Mitochondrion matrix

Conjugation

Non-conjugated

Modification

Unmodified

Research Field

Tags & Cell Markers

Product Categories

Primary Antibody; Mouse Monoclonal Antibody

Clonality

Monoclonal

Host

Mouse

Reactivity

Human, Mouse, Rat

SwissProt ID

SwissProt: P10809 Human;SwissProt: P63038 Mouse;SwissProt: P63039 Rat

Dilution Ratio

WB: 1:500-1:2,000 ;ICC: 1:100 ;IHC-P: 1:50-1:1,000

  • Western blot analysis of extracts from Hela(lane 2(20μg), HepG2(lane 3(20μg), Jurkat(lane 4(20μg) ) and NIH3T3(lane 5(20μg) ) using HSP60 (HY-P80184) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80184, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application

WB, ICC/IF, IHC-P

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG1

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human HSP60.AA range:524-573.

Documentation

HSP60 Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HSP60 Antibody
Cat. No.:
HY-P80184
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