Human IgM Antibody (YA1062)

Human IgM Antibody (YA1062) is a mouse-derived non-conjugated IgG2a antibody (Clone NO.: YA1062), targeting Human IgM. Human IgM Antibody (YA1062) can be used for ELISA, IHC-P experiment in human background.

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region related gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of related genes: V related genes (see MIM 147070), joining (J) related genes (see MIM 147010), and diversity (D) related genes (see MIM 146910). The C region related genes are clustered downstream of the V region related genes within the heavy chain locus on chromosome 14. The IGHM related gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region related genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).

3507

-

Affinity Purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

P01871

IHC: 1/50-1/100;ELISA: 1/10000

ELISA, IHC-P

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Purified recombinant full length of human IgM heavy chain protein expressed in E.coli.