2. Antibodies
  3. Primary Antibodies
  4. Polyclonal Antibodies
  5. BNSP Antibody

BNSP Antibody

Cat. No.: HY-P80969
COA User Guide for Antibodies

IBSP Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 35 kDa, targeting to IBSP. It can be used for WB, IHC-P assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $75 In-stock
50 μL $190 In-stock
100 μL $310 In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

IBSP Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 35 kDa, targeting to IBSP. It can be used for WB, IHC-P assays with tag free, in the background of Human, Mouse.
Gene ID

3381
Synonyms
integrin binding sialoprotein; BSP; BNSP; SP-II; BSP-II; IBSP; Bone sialoprotein II; Cell binding sialoprotein; Integrin binding sialoprotein; SIAL_HUMAN; Bone sialoprotein 2; Cell-binding sialoprotein; Integrin-binding sialoprotein.
Molecular Weight

Predicted band size: 35 kDa;Observed band size: 55 kDa
Conjugation

Non-conjugated
Modification

Unmodified
Product Categories

Primary Antibody; Rabbit Polyclonal Antibody
Clonality

Polyclonal
Host

Rabbit
Reactivity

Mouse, Rat
Predicted Reactivity

Human

Note: The predicted reactivity is for reference only, not as a product performance guarantee.
SwissProt ID

P21815
Dilution Ratio

WB: 1:500-2000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500;ELISA: 1:5000-10000;

  • Western blot analysis of extracts from M-Heart (lane 1) and C2C12 (lane 2) and HepG2 (lane 3) and 293T (lane 4) using BNSP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application

WB, IHC-P, IHC-F, ICC/IF, ELISA
Appearance

Liquid
Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

KLH conjugated synthetic peptide derived from human Bone sialoprotein II: 21-120/317
Documentation

COA (191 KB)

User Guide for Antibodies (1077 KB)

BNSP Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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