Ki-67 Antibody

Ki-67 Antibody is an unconjugated, approximately 358 kDa, rabbit-derived, anti-Ki-67 polyclonal antibody. Ki-67 Antibody can be used for: IHC-P, IHC-F, ICC, IF expriments in human background without labeling.

17345

Predicted band size: 358kDa

affinity purified

Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Non-conjugated

Unmodified

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

Q91VE6

IHC-P: 1:100-500;IHC-F: 1:100-500;ICC: 1:100;IF: 1:100-500

• 
  Western blot analysis of extracts from M-liver (lane 1) using Ki-67 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

IHC-P, IHC-F, ICC/IF

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from mouse Ki-67: 1231-1330/2938