Phospho-CDK2 (Tyr15) Antibody

Phospho-CDK2 (Tyr15) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 34 kDa, targeting to Phospho-CDK2 (Tyr15). It can be used for WB,IP assays with tag free, in the background of Human, Hamster, Rat.

CDK2: This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Free

1017

Predicted band size: 34 kDa

affinity purified

Non-conjugated

Phosphorylated

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Hamster, Rat

P24941

WB: 1:500-1:1,000 ;IP: 1:20

• 
  Western blot analysis of extracts from C6(lane 2(20μg) or lane 3(40μg)), using Phospho-CDK2 (HY-P80798) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80798, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Tyr15 of Human Cdk2.The exact sequence is proprietary to MCE.