Phospho-HSF1 (Ser303/Ser307) Antibody

Phospho-HSF1 (Ser303/Ser307) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 57 kDa, targeting to Phospho-HSF1 (Ser303/Ser307). It can be used for WB,IHC-P assays with tag free, in the background of Human.

HSF1: The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]

Free

3297

Predicted band size: 57 kDa

affinity purified

Non-conjugated

Phosphorylated

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

Q00613

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Western blot analysis of extracts from HeLa (lane 2(20μg)), K562 (lane 3(20μg)) and 293 (lane 5(20μg)) using phospho-HSF1 (Ser303/Ser307) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser303/Ser307 of Human HSF1.The exact sequence is proprietary to MCE.