Phospho-IKB alpha (Ser32) Antibody (YA183)

Phospho-IKB alpha (Ser32) Antibody (YA183) is a non-conjugated and Rabbit origined monoclonal antibody about 36 kDa, targeting to Phospho-IKB alpha(S32). It can be used for WB,ICC,IP assays with tag free, in the background of Human, Mouse.

Free

Predicted band size: 36 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Phosphorylated

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

SwissProt: P25963 Human;SwissProt: Q9Z1E3 Mouse

WB: 1:1,000 ;ICC: 1:50-1:200 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) ) and Hela(lane 3(20μg), Human TNF-alpha treated (HY-P7058, 20 ng/mL Human TNF-α, 2hrs) as indicated, using Phospho-IKB alpha (Ser32) (HY-P80446) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling Phospho-IKB alpha (Ser32) with Phospho-IKB alpha (Ser32) antibody (HY-P80466) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-IKB alpha (Ser32) antibody (HY-P80466) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser32 of Human IKB alpha.The exact sequence is proprietary to MCE.