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  4. Phospho-NF-KB p65 (Thr254) Antibody

Phospho-NF-KB p65 (Thr254) Antibody

Cat. No.: HY-P80840
COA User Guide for Antibodies

Phospho-NF-KB p65 (Thr254) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 60 kDa, targeting to Phospho-NF-KB p65 (Thr254). It can be used for WB,IHC-P,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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Description

Phospho-NF-KB p65 (Thr254) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 60 kDa, targeting to Phospho-NF-KB p65 (Thr254). It can be used for WB,IHC-P,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

Background

NF-KB p65: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Tag

Free

Gene ID

5970

Synonyms
NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB
Molecular Weight

Predicted band size: 60 kDa

Purity

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Research Field

Cell Biology

Product Categories

Primary Antibody; Rabbit Polyclonal Antibody

Clonality

Polyclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

SwissProt ID

Q04206

Dilution Ratio

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200

Application

WB, IHC-P, ICC/IF

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr 254 of Human NFκB-p65.The exact sequence is proprietary to MCE.

Documentation

Phospho-NF-KB p65 (Thr254) Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Phospho-NF-KB p65 (Thr254) Antibody
Cat. No.:
HY-P80840
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