TGF alpha Antibody

TGF alpha Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 17 kDa, targeting to TGF alpha. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human.

TGF alpha: This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Free

7039

Predicted band size: 17 kDa

affinity purified

Non-conjugated

Unmodified

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P01135

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IP: 1:20

• 
  Western blot analysis of extracts from K562(lane 2(20μg)) and HepG2(lane 3(20μg)), using TGF alpha (HY-P80911) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80911, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human TGF alpha.The exact sequence is proprietary to MCE.