Toll-Like Receptor 3 Antibody

Toll-Like Receptor 3 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 105 kDa, targeting to Toll-Like Receptor 3 . It can be used for WB, IHC-P, IHC-F, IF, ELISA assays with tag free, in the background of Human, Mouse, Rat.

Toll-Like Receptor 3 (2F10): The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It thus plays a role in host defense against multiple viruses. [provided by RefSeq, Jul 2021]

Free

7098

Predicted band size: 105 kDa;Observed band size: 130 kDa

affinity purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

O15455

WB: 1:500-2000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500;ELISA: 1:5000-10000;

• 
  Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and M-Intestine (lane 3) using TLR3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, IHC-F, ICC/IF, ELISA

Liquid

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human TLR3: 801-905/905.