Toll-Like Receptor 4 Antibody

Toll-Like Receptor 4 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 96 kDa, targeting to Toll-Like Receptor 4. It can be used for WB,FC assays with tag free, in the background of Human.

Toll-Like Receptor 4: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020]

Free

7099

Predicted band size: 96 kDa;Observed band size: 96-130 kDa

affinity purified

Non-conjugated

Unmodified

Immunology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

O00206

WB: 1:500-1:1,000 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from 3T3 (lane 1) and Intestine (lane 2) using Toll-like receptor 4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, FC

Liquid

Supplied in 1*PBS (pH 7.3). Preservative: 0.09% sodium azide or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human TLR4.AA range:669-698 or KLH conjugated synthetic peptide derived from human TLR4: 121-200/839.