TriMethyl-Histone H3 (Lys27) Antibody

TriMethyl-Histone H3 (Lys27) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 15 kDa, targeting to TriMethyl-Histone H3 (Lys27). It can be used for WB,IHC-F,IHC-P,ICC/IF,IP,ChIP assays with tag free, in the background of Human, Rat.

TriMethyl-Histone H3 (Lys27): Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Free

8350

Predicted band size: 15 kDa

affinity purified

Non-conjugated

Methylated

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

P68431

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;IP: 1:20 ;ChIP: 1:20

WB, IHC-F, IHC-P, ICC/IF, IP, ChIP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to residues surrounding Lys27 of Human Histone H3.The exact sequence is proprietary to MCE.