ZAP70 Antibody (YP4051)

Cat. No.: HY-P80524: FAQs
Data Sheet SDS COA User Guide for Antibodies

ZAP70 Antibody (YP4051) is a non-conjugated and Rabbit origined monoclonal antibody about 70 kDa, targeting to ZAP70. It can be used for WB,ICC,IHC-P,FC,IP assays with tag free, in the background of Human.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	ZAP70 Antibody (YP4051) is a non-conjugated and Rabbit origined monoclonal antibody about 70 kDa, targeting to ZAP70. It can be used for WB,ICC,IHC-P,FC,IP assays with tag free, in the background of Human.
Tag	Free
Molecular Weight	Predicted band size: 70 kDa;Observed band size: 74 kDa
Purity	Protein A affinity purified.
Subcellular Location	Cytoplasm. Cell membrane.
Conjugation	Non-conjugated
Modification	Unmodified
Research Field	Immunology
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human
SwissProt ID	SwissProt: P43403 Human
Dilution Ratio	WB: 1:500-1:1,000 ;ICC: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: 1:10-1:50 Western blot analysis of extracts from Jurkat(lane 2(20μg) ) and Jurkat(lane 3(40μg) ) using ZAP70 (HY-P80524) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80524, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application	WB, ICC/IF, IHC-P, FC, IP
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Human ZAP70.AA range:570-619.
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

ZAP70 Antibody (YP4051) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ZAP70 Antibody (YP4051)

Biological Activity

Technical Parameters

Properties

Documentation

ZAP70 Antibody (YP4051) Related Classifications

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