2. Cell Cycle/DNA Damage
  3. CDK
  4. Atirmociclib

Atirmociclib  (Synonyms: PF-07220060; CDK4/6-IN-6)

Cat. No.: HY-139450 Purity: 99.87%
COA Handling Instructions

Molarity Calculator

Atirmociclib (PF-07220060; example A94) is a potent CDK4/CDK6 inhibitor with a Ki of 0.6 nM and 13.9 nM for CDK4/Cyclin D1 and CDK6/Cyclin D3, respectively.

For research use only. We do not sell to patients.

Atirmociclib Chemical Structure

Atirmociclib Chemical Structure

CAS No. : 2380321-51-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 306 In-stock
Solution
10 mM * 1 mL in DMSO USD 306 In-stock
Solid
1 mg USD 142 In-stock
5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 950 In-stock
50 mg USD 1450 In-stock
100 mg USD 2250 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Atirmociclib Related Antibodies

Top Publications Citing Use of Products

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Atirmociclib (PF-07220060; example A94) is a potent CDK4/CDK6 inhibitor with a Ki of 0.6 nM and 13.9 nM for CDK4/Cyclin D1 and CDK6/Cyclin D3, respectively[1].

IC50 & Target[1]

Cdk4/cyclin D1

0.6 nM (Ki)

cdk6/cyclin D3

13.9 nM (Ki)

In Vitro

Atirmociclib (PF-07220060; example A94) has IC50s of 38.5 nM and 144.9 nM for CDK4/Cyclin D1 and CDK6/Cyclin D3 using phospho-Rb S795 ELISA assays in JEKO-1 and MV4-1 1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Atirmociclib Related Antibodies
Molecular Weight

463.93

Formula

C22H27ClFN5O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(N1C2=CC(C3=NC(N[C@H]4[C@@H](COCC4)O)=NC=C3Cl)=CC(F)=C2N=C1C(C)(O)C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (538.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1555 mL 10.7775 mL 21.5550 mL
5 mM 0.4311 mL 2.1555 mL 4.3110 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.87%

COA (248 KB) HNMR (288 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1555 mL 10.7775 mL 21.5550 mL 53.8874 mL
5 mM 0.4311 mL 2.1555 mL 4.3110 mL 10.7775 mL
10 mM 0.2155 mL 1.0777 mL 2.1555 mL 5.3887 mL
15 mM 0.1437 mL 0.7185 mL 1.4370 mL 3.5925 mL
20 mM 0.1078 mL 0.5389 mL 1.0777 mL 2.6944 mL
25 mM 0.0862 mL 0.4311 mL 0.8622 mL 2.1555 mL
30 mM 0.0718 mL 0.3592 mL 0.7185 mL 1.7962 mL
40 mM 0.0539 mL 0.2694 mL 0.5389 mL 1.3472 mL
50 mM 0.0431 mL 0.2155 mL 0.4311 mL 1.0777 mL
60 mM 0.0359 mL 0.1796 mL 0.3592 mL 0.8981 mL
80 mM 0.0269 mL 0.1347 mL 0.2694 mL 0.6736 mL
100 mM 0.0216 mL 0.1078 mL 0.2155 mL 0.5389 mL
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Atirmociclib Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Atirmociclib2380321-51-5PF-07220060 CDK4/6-IN-6PF07220060PF 07220060CDKCyclin dependent kinaseCyclinD1D3Inhibitorinhibitorinhibit

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