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  3. ATTO 488 carboxylic acid

ATTO 488 carboxylic acid is a new fluorescent label based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 carboxylic acid is a carboxylic acid derivative of ATTO 488, which can be used to label proteins or antibodies.

For research use only. We do not sell to patients.

ATTO 488 carboxylic acid Chemical Structure

ATTO 488 carboxylic acid Chemical Structure

CAS No. : 1443553-08-9

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Based on 1 publication(s) in Google Scholar

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Description

ATTO 488 carboxylic acid is a new fluorescent label based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 carboxylic acid is a carboxylic acid derivative of ATTO 488, which can be used to label proteins or antibodies.

Molecular Weight

589.59

Formula

C25H23N3O10S2

CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=C(CCCN(C)C(C1=C(C2=C(C=CC(N)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC=C1)=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (169.61 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6961 mL 8.4805 mL 16.9609 mL
5 mM 0.3392 mL 1.6961 mL 3.3922 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.6961 mL 8.4805 mL 16.9609 mL 42.4023 mL
5 mM 0.3392 mL 1.6961 mL 3.3922 mL 8.4805 mL
10 mM 0.1696 mL 0.8480 mL 1.6961 mL 4.2402 mL
15 mM 0.1131 mL 0.5654 mL 1.1307 mL 2.8268 mL
20 mM 0.0848 mL 0.4240 mL 0.8480 mL 2.1201 mL
25 mM 0.0678 mL 0.3392 mL 0.6784 mL 1.6961 mL
30 mM 0.0565 mL 0.2827 mL 0.5654 mL 1.4134 mL
40 mM 0.0424 mL 0.2120 mL 0.4240 mL 1.0601 mL
50 mM 0.0339 mL 0.1696 mL 0.3392 mL 0.8480 mL
60 mM 0.0283 mL 0.1413 mL 0.2827 mL 0.7067 mL
80 mM 0.0212 mL 0.1060 mL 0.2120 mL 0.5300 mL
100 mM 0.0170 mL 0.0848 mL 0.1696 mL 0.4240 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
ATTO 488 carboxylic acid
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HY-D1305
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